brucella abortus antibody  (Novus Biologicals)

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles.

doi: 10.1080/21691401.2019.1626403

Figure Lengend Snippet: Figure 3. (A) Visual results of the colorimetric assay by serial dilutions of the B. abortus from 1.5 108 to 1.5 10 cfu mL1, by reducing the concentration of bac- teria the intensity of the color decreases; (B) A linear dependence between the dye absorbance and logarithm of B. abortus concentration.

Article Snippet: Polyclonal antibody against Brucella abortus was purchased from Biorbyt, orb10564 (Biorbyt, USA).

Techniques: Colorimetric Assay, Concentration Assay

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles.

doi: 10.1080/21691401.2019.1626403

Figure Lengend Snippet: Figure 2. Condition optimization results for rapid colorimetric detection of B. abortus, (A) PMNPs and blue-SiNP-NH2 optimization results, optimum concentration of PMNPs 5 mg and blue-SiNP-NH210 mg were determined; (B) 5 lL was the optimized concentration of the antibody; (C) Time optimization results, best time for conjugation of antibody with EDC/NHS complex was calculated as 240 min; (D) Optimized time for conjugated of bacteria with nanoparticles was 60 min.

Article Snippet: Polyclonal antibody against Brucella abortus was purchased from Biorbyt, orb10564 (Biorbyt, USA).

Techniques: Concentration Assay, Conjugation Assay, Bacteria